• Immunohistochemistry analysis of paraffin-embedded mouse heart using ACADL antibody (STJ22475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using ACADL antibody (STJ22475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using ACADL antibody (STJ22475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ACADL antibody (STJ22475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACADL antibody (31-210) (STJ22475)

SKU:
STJ22475

€140.63 - €655.63
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACADL
Gene ID: 33
Uniprot ID: ACADL_HUMAN
Immunogen Region: 31-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 31-210 of human ACADL (NP_001599.1).
Immunogen Sequence: GGEERLETPSAKKLTDIGIR RIFSPEHDIFRKSVRKFFQE EVIPHHSEWEKAGEVSREVW EKAGKQGLLGVNIAEHLGGI GGDLYSAAIVWEEQAYSNCS GPGFSIHSGIVMSYITNHGS EEQIKHFIPQMTAGKCIGAI AMTEPGAGSDLQGIKTNAKK DGSDWILNGSKVFISNGSLS
Post Translational Modifications Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.
Function Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.
Protein Name Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Lcad
Database Links Reactome: R-HSA-77285
Reactome: R-HSA-77288
Reactome: R-HSA-77310
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Lcad antibody
Anti-ACADL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance