• Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 125ng/ml 15', using Acetyl-CoA Carboxylase Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Acetyl-CoA Carboxylase Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of NIH-3T3 cells using ACC Alpha Polyclonal Antibody
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of 3T3 cells using ACC Alpha Polyclonal Antibody

Anti-ACACA antibody (46-95 aa) (STJ91439)

SKU:
STJ91439

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Cow/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-CoA carboxylase 1 (46-95 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACACA
Gene ID: 31
Uniprot ID: ACACA_HUMAN
Immunogen Region: 46-95 aa
Specificity: ACC Alpha Polyclonal Antibody detects endogenous levels of ACC Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Acetyl-CoA Carboxylase at the amino acid range 46-95
Post Translational Modifications Phosphorylation on Ser-1263 is required for interaction with BRCA1. Phosphorylation at Ser-80 by AMPK inactivates enzyme activity. The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Protein Name Acetyl-Coa Carboxylase 1
Acc1
Acetyl-Coenzyme A Carboxylase Alpha
Acc-Alpha
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-196780
Reactome: R-HSA-200425
Reactome: R-HSA-2426168
Reactome: R-HSA-3371599
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coa Carboxylase 1 antibody
Anti-Acc1 antibody
Anti-Acetyl-Coenzyme A Carboxylase Alpha antibody
Anti-Acc-Alpha antibody
Anti-ACACA antibody
Anti-ACAC antibody
Anti-ACC1 antibody
Anti-ACCA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance