| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ABHD14B (188-200) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 24kDa band observed in Human, Mouse, and Rat Liver lysates (calculated MW of 22.3kDa according to NP_116139.1). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ABHD14B |
| Gene ID: | 84836 |
| Uniprot ID: | ABHEB_HUMAN |
| Immunogen Region: | 188-200 |
| Accession Number: | NP_116139.1 |
| Specificity: | Reported variants represent identical protein: NP_116139.1, NP_001139786.1. This antibody is expected to NOT cross-react with ABHD14A. |
| Immunogen Sequence: | HPCYLDKPEEWHT |
| Function | Acts as an atypical protein-lysine deacetylase in vitro. Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues. Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate. May activate transcription. |
| Protein Name | Putative Protein-Lysine Deacylase Abhd14bAlpha/Beta Hydrolase Domain-Containing Protein 14bAbhydrolase Domain-Containing Protein 14bCcg1-Interacting Factor B |
| Database Links | Reactome: R-HSA-156584 |
| Cellular Localisation | CytoplasmNucleusPredominantly Cytoplasmic |
| Alternative Antibody Names | Anti-Putative Protein-Lysine Deacylase Abhd14b antibodyAnti-Alpha/Beta Hydrolase Domain-Containing Protein 14b antibodyAnti-Abhydrolase Domain-Containing Protein 14b antibodyAnti-Ccg1-Interacting Factor B antibodyAnti-ABHD14B antibodyAnti-CIB antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98597/382525/STJ119808_1__29834.1713150947.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
