Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-ABHD14B (188-200) is suitable for use in ELISA and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 24kDa band observed in Human, Mouse, and Rat Liver lysates (calculated MW of 22.3kDa according to NP_116139.1). Recommended concentration: 0.3-1µg/ml. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | ABHD14B |
Gene ID: | 84836 |
Uniprot ID: | ABHEB_HUMAN |
Immunogen Region: | 188-200 |
Accession Number: | NP_116139.1 |
Specificity: | Reported variants represent identical protein: NP_116139.1, NP_001139786.1. This antibody is expected to NOT cross-react with ABHD14A. |
Immunogen Sequence: | HPCYLDKPEEWHT |
Function | Acts as an atypical protein-lysine deacetylase in vitro. Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues. Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate. May activate transcription. |
Protein Name | Putative Protein-Lysine Deacylase Abhd14bAlpha/Beta Hydrolase Domain-Containing Protein 14bAbhydrolase Domain-Containing Protein 14bCcg1-Interacting Factor B |
Database Links | Reactome: R-HSA-156584 |
Cellular Localisation | CytoplasmNucleusPredominantly Cytoplasmic |
Alternative Antibody Names | Anti-Putative Protein-Lysine Deacylase Abhd14b antibodyAnti-Alpha/Beta Hydrolase Domain-Containing Protein 14b antibodyAnti-Abhydrolase Domain-Containing Protein 14b antibodyAnti-Ccg1-Interacting Factor B antibodyAnti-ABHD14B antibodyAnti-CIB antibody |
Information sourced from Uniprot.org