• Western blot analysis of various tissues from Arabidopsis thaliana, using ABF1 Rabbit polyclonal antibody (STJ11104955) at 1:600 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ABF1 antibody (100-200 aa) (STJ11104955)

SKU:
STJ11104955

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 100-200 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of Arabidopsis thaliana ABF1. (NP_564551.1).
Immunogen Sequence: PRTLSQKTVDEVWKYLNSKE GSNGNTGTDALERQQTLGEM TLEDFLLRAGVVKEDNTQQN ENSSSGFYANNGAAGLEFGF GQPNQNSISFNGNNSSMIMN Q
Background Identified as a protein that binds to abscisic acid response elements. May mediate transcriptional regulation of ABA responses.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance