Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4ยฐC overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-ABCG5 antibody (250-330 aa) (STJ190586)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 250-330
STJ190586
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General Information

Short DescriptionRabbit polyclonal anti-ATP-binding cassette sub-family G member 5 (250-330 aa) for use in WB, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
ELISA 1:5000
IF 1:100-300
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolABCG5
Gene ID64240
Uniprot IDABCG5_HUMAN
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 250-330
Immunogen Region250-330 aa
SpecificityABCG5 Polyclonal Antibody detects endogenous levels of protein.

Additional Info

Post Translational Modifications N-glycosylated.
Function ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.
Protein Name Atp-Binding Cassette Sub-Family G Member 5
Sterolin-1
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-5679090
Reactome: R-HSA-5679096
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family G Member 5 antibody
Anti-Sterolin-1 antibody
Anti-ABCG5 antibody

Information sourced from Uniprot.org

Citations

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