• Immunofluorescence analysis of Rat lung using ABCG1 antibody (STJ22465) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ABCG1 Rabbit polyclonal antibody (STJ22465) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ABCG1 Rabbit polyclonal antibody (STJ22465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ABCG1 antibody (6-120) (STJ22465)

SKU:
STJ22465

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCG1 (6-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCG1
Gene ID: 9619
Uniprot ID: ABCG1_HUMAN
Immunogen Region: 6-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 6-120 of human ABCG1 (NP_004906.3).
Immunogen Sequence: AAFSVGTAMNASSYSAEMTE PKSVCVSVDEVVSSNMEATE TDLLNGHLKKVDNNLTEAQR FSSLPRRAAVNIEFRDLSYS VPEGPWWRKKGYKTLLKGIS GKFNSGELVAIMGPS
Tissue Specificity Expressed in several tissues. Expressed in macrophages.expression is increased in macrophages from patients with Tangier disease.
Post Translational Modifications Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
Function Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.
Protein Name Atp-Binding Cassette Sub-Family G Member 1
Atp-Binding Cassette Transporter 8
White Protein Homolog
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-8964058
Reactome: R-HSA-9029569
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Cell Membrane
Predominantly Localized In The Intracellular Compartments Mainly Associated With The Endoplasmic Reticulum (Er) And Golgi Membranes
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family G Member 1 antibody
Anti-Atp-Binding Cassette Transporter 8 antibody
Anti-White Protein Homolog antibody
Anti-ABCG1 antibody
Anti-ABC8 antibody
Anti-WHT1 antibody

Information sourced from Uniprot.org

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