Anti-AATF antibody (10-59 aa) (STJ92259)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human the amino acidTF at the amino acid range 10-59
STJ92259
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General Information

Short DescriptionRabbit polyclonal anti-Protein AATF (10-59 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolAATF
Gene ID26574
Uniprot IDAATF_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human the amino acidTF at the amino acid range 10-59
Immunogen Region10-59 aa
SpecificityChe-1 Polyclonal Antibody detects endogenous levels of Che-1 protein.

Additional Info

Post Translational Modifications Hyperphosphorylated during the G1/S phase transition.
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Protein Name Protein Aatf
Apoptosis-Antagonizing Transcription Factor
Rb-Binding Protein Che-1
Database Links Reactome: R-HSA-193648
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Protein Aatf antibody
Anti-Apoptosis-Antagonizing Transcription Factor antibody
Anti-Rb-Binding Protein Che-1 antibody
Anti-AATF antibody
Anti-CHE1 antibody
Anti-DED antibody
Anti-HSPC277 antibody

Information sourced from Uniprot.org

Citations

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