![Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186326/408068/STJ11107130_1__83772.1744894494.jpg?c=1 "Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and AARS2 knockdown (KD) 293T cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186326/408069/STJ11107130_2__39061.1744894494.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AARS2 knockdown (KD) 293T cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
![Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186326/408068/STJ11107130_1__83772.1744894494.jpg?c=1 "Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
Anti-AARS2 antibody (491-620 aa) (STJ11107130)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AARS2 (491-620 aa) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AARS2 |
| Gene ID | 57505 |
| Uniprot ID | SYAM_HUMAN |
| Immunogen Region | 491-620 aa |
| Immunogen Sequence | GLWLDVHALGELQRQGVPPT DDSPKYNYSLRPSGSYEFGT CEAQVLQLYTEDGTAVASVG KGQRCGLLLDRTNFYAEQGG QASDRGYLVRAGQEDVLFPV ARAQVCGGFILHEAVAPECL RLGDQVQLHV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 491-620 of human AARS2 (NP_065796.2). |
Additional Info
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. In presence of high levels of lactate, also acts as a protein lactyltransferase that mediates lactylation of lysine residues in target proteins, such as CGAS. Acts as an inhibitor of cGAS/STING signaling by catalyzing lactylation of CGAS, preventing the formation of liquid-like droplets in which CGAS is activated. |
| Protein Name | Alanine--Trna Ligase - MitochondrialAlanyl-Trna SynthetaseAlarsProtein Lactyltransferase Aars2 |
| Database Links | Reactome: R-HSA-379726 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Alanine--Trna Ligase - Mitochondrial antibodyAnti-Alanyl-Trna Synthetase antibodyAnti-Alars antibodyAnti-Protein Lactyltransferase Aars2 antibodyAnti-AARS2 antibodyAnti-AARSL antibodyAnti-KIAA1270 antibody |
Information sourced from Uniprot.org