![Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186326/408068/STJ11107130_1__83772.1744894494.jpg?c=1 "Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and AARS2 knockdown (KD) 293T cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186326/408069/STJ11107130_2__39061.1744894494.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AARS2 knockdown (KD) 293T cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
![Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186326/408068/STJ11107130_1__83772.1744894494.jpg?c=1 "Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | AARS2 |
| Gene ID: | 57505 |
| Uniprot ID: | SYAM_HUMAN |
| Immunogen Region: | 491-620 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 491-620 of human AARS2 (NP_065796.2). |
| Immunogen Sequence: | GLWLDVHALGELQRQGVPPT DDSPKYNYSLRPSGSYEFGT CEAQVLQLYTEDGTAVASVG KGQRCGLLLDRTNFYAEQGG QASDRGYLVRAGQEDVLFPV ARAQVCGGFILHEAVAPECL RLGDQVQLHV |
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
| Protein Name | Alanine--Trna Ligase - MitochondrialAlanyl-Trna SynthetaseAlars |
| Database Links | Reactome: R-HSA-379726 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Alanine--Trna Ligase - Mitochondrial antibodyAnti-Alanyl-Trna Synthetase antibodyAnti-Alars antibodyAnti-AARS2 antibodyAnti-AARSL antibodyAnti-KIAA1270 antibody |
Information sourced from Uniprot.org
