| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-AARS2 (478-489) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: In transfected 143B transiently expressing Human AARS2 a band of approx. 100kDa is observed. This band is barely visible in the non-transfected 143B. The calculated molecular size is 1 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | AARS2 |
| Gene ID: | 57505 |
| Uniprot ID: | SYAM_HUMAN |
| Immunogen Region: | 478-489 |
| Accession Number: | NP_065796.1 |
| Immunogen Sequence: | QHRARQAEPVQK |
| Protein Name | Alanine--Trna Ligase - MitochondrialAlanyl-Trna SynthetaseAlars |
| Database Links | Reactome: R-HSA-379726 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Alanine--Trna Ligase - Mitochondrial antibodyAnti-Alanyl-Trna Synthetase antibodyAnti-Alars antibodyAnti-AARS2 antibodyAnti-AARSL antibodyAnti-KIAA1270 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186326/408068/STJ11107130_1__83772.1744894494.jpg?c=1 "Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (STJ11107130) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.")
