• Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (STJ11101211) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-2019-nCoV antibody [S1MR] (STJ11101211)

SKU:
STJ11101211

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Host: Rabbit
Applications: WB/FC/IF/IP
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SARS-COV-2 Spike is suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:1000-1:5000
WB 1:2000-1:10000
FC 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at 4°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Recombinant fusion protein of SARS-CoV-2 Spike S1.
Immunogen Sequence: Email for sequence
Background The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

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