| Host: | Mouse |
| Applications: | ICC/IHC-F/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-14-3-3 protein eta (Full-Length) for use in ICC, IHC-F and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 3G12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | IgG |
| Dilution Range: | WB 1:1000-1:5000IHC 1:500-1:1000ICC 1:500-1:1000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
| Gene Symbol: | YWHAH |
| Gene ID: | 7533 |
| Uniprot ID: | 1433F_HUMAN |
| Immunogen Region: | Full-Length |
| Specificity: | Human 14-3-3 ETA protein. |
| Immunogen: | Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli. |
| Immunogen Sequence: | Human |
| Tissue Specificity | Expressed mainly in the brain and present in other tissues albeit at lower levels. |
| Post Translational Modifications | Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. |
| Protein Name | 14-3-3 Protein EtaProtein As1 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-75035Reactome: R-HSA-9735871Reactome: R-HSA-9755779Reactome: R-HSA-9856649 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-14-3-3 Protein Eta antibodyAnti-Protein As1 antibodyAnti-YWHAH antibodyAnti-YWHA1 antibody |
Information sourced from Uniprot.org
