| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Angiopoietin-like 4 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:2, 500-1:5, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Angptl4 |
| Gene ID: | 57875 |
| Uniprot ID: | ANGL4_MOUSE |
| Immunogen: | Purfied protein. |
| Tissue Specificity | Detected in liver and kidney. Predominantly expressed in adipose tissue and is strongly up-regulated by fasting in white adipose tissue and liver. More abundant in areas of lower flow stress in the inner curvature compared to the outer curvature regions of the aorta (at protein level). |
| Post Translational Modifications | N-glycosylated. ANGPTL4 N-terminal chain: Forms disulfide-linked dimers and tetramers. Cleaved into a smaller N-terminal chain and a larger chain that contains the fibrinogen C-terminal domain.both cleaved and uncleaved forms are detected in the extracellular space. The cleaved form is not present within the cell. |
| Function | Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis (Probable). ANGPTL4 N-terminal chain: Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein. |
| Peptide Name | Angiopoietin-Related Protein 4 425o18-1 Angiopoietin-Like Protein 4 Fasting-Induced Adipose Factor Hepatic Fibrinogen/Angiopoietin-Related Protein Hfarp Secreted Protein Bk89 Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain |
| Database Links | Reactome: R-MMU-8963889 Reactome: -MMU-9762292 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix The Unprocessed Form Interacts With The Extracellular Matrix This May Constitute A Dynamic Reservoir A Regulatory Mechanism Of The Bioavailability Of Angptl4 |
| Alternative Peptide Names | Angiopoietin-Related Protein 4 protein 425o18-1 protein Angiopoietin-Like Protein 4 protein Fasting-Induced Adipose Factor protein Hepatic Fibrinogen/Angiopoietin-Related Protein protein Hfarp protein Secreted Protein Bk89 Cleaved Into - Angptl4 N-Terminal Chain - Angptl4 C-Terminal Chain protein Angptl4 protein Farp protein Fiaf protein Ng27 protein |
Information sourced from Uniprot.org