| Short Description : | Recombinant-African swine fever virus P30-NO-Tag protein was developed in e.coli using the region Ile86-Lys194. For use in research applications. |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Supplied as 0.22 Mu m filtered solution in PBS, 300 mM NaCl, 10% glycerol, (pH 7.4). |
| Storage Instruction: | Store at-70 °C. This product is stable at ≤-70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
| Determination Method: | Please contact us for more information. |
| Immunogen: | Recombinant Recombinant African swine fever virus P30 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ile86-Lys194) of African swine fever virus P30 (Accession #P34204) fused with no tag. |
| Immunogen Region: | Ile86-Lys194 |
| Immunogen Sequence: | ILHVLFEEETESSASSENIH EKNDNETNECTSSFETLFEQ EPSSEVPKDSKLYMLAQKTV QHIEQYGKAPDFNKVIRAHN FIQTIYGTPLKEEEKEVVRL MVIKLLKKK |
| Background | ASFV structural protein p30 is a significant immunogen reported to induce neutralizing antibodies against it. The p30 interacts with heterologous nuclear ribonucleoprotein K (hnRNPK) in the host cell and could downregulate the host cell mRNA translation (Hernaez et al., 2008). Moreover, p30 is an excellent diagnostic marker for ASFV, and anti-p30 antibodies can be detected as early as eight days post-infection (Gómez-Puertas et al., 1998).We and others previously showed the epitopic domains of the p30 protein, which could potentially generate the host immune response (Afonso et al., 1992; Imdhiyas et al., 2022; Petrovan et al., 2019). |
Information sourced from Uniprot.org