| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
YAP1 Positive Control for STJ506331 is synthetically produced from the 300-350 sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
YAP1 |
| Gene ID: |
10413 |
| Uniprot ID: |
YAP1_HUMAN |
| Immunogen Region: |
300-350 |
| Specificity: |
This is positive control is recommended for use in combination with YAP1 antibody STJ506331. |
| Tissue Specificity | Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). |
| Post Translational Modifications | Phosphorylated by LATS1 and LATS2.leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1.leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes.in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1. Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase. |
| Function | Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). |
| Peptide Name | Transcriptional Coactivator Yap1Yes-Associated Protein 1Protein Yorkie HomologYes-Associated Protein Yap65 Homolog |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-2028269Reactome: R-HSA-2032785Reactome: R-HSA-8939236Reactome: R-HSA-8940973Reactome: R-HSA-8951671Reactome: R-HSA-9619665Reactome: R-HSA-9796292 |
| Cellular Localisation | CytoplasmNucleusCell JunctionBoth Phosphorylation And Cell Density Can Regulate Its Subcellular LocalizationPhosphorylation Sequesters It In The Cytoplasm By Inhibiting Its Translocation Into The NucleusAt Low DensityPredominantly Nuclear And Is Translocated To The Cytoplasm At High DensityPtpn14 Induces Translocation From The Nucleus To The CytoplasmLocalized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm At The Blastocyst And Epiblast Stages |
| Alternative Peptide Names | Transcriptional Coactivator Yap1 proteinYes-Associated Protein 1 proteinProtein Yorkie Homolog proteinYes-Associated Protein Yap65 Homolog proteinYAP1 proteinYAP65 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
