| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Topoisomerase II alpha Blocking Peptide is synthetically produced from the 1220-1270 sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | TOP2A |
| Gene ID: | 7153 |
| Uniprot ID: | TOP2A_HUMAN |
| Immunogen Region: | 1220-1270 |
| Immunogen: | Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein. |
| Tissue Specificity | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas. |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Peptide Name | Dna Topoisomerase 2-Alpha Dna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277 Reactome: R-HSA-4615885 |
| Cellular Localisation | Cytoplasm Nucleus Nucleoplasm Nucleolus |
| Alternative Peptide Names | Dna Topoisomerase 2-Alpha protein Dna Topoisomerase Ii - Alpha Isozyme protein TOP2A protein TOP2 protein |
Information sourced from Uniprot.org