| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Syncytin A Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis. Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and trachea. Both mRNA and protein levels are significantly increased in the brain of individuals with multiple sclerosis, particularly in astrocytes and microglia. |
| Post Translational Modifications | Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as an inactive precursor that is heavily N-glycosylated and processed likely by furin in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence KR-X-KR-R. The intracytoplasmic tail cleavage by the viral protease that is required for the fusiogenic activity of some retroviruses envelope proteins seems to have been lost during evolution. The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion. |
| Function | This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein (SU) mediates receptor recognition, while the transmembrane protein (TM) acts as a class I viral fusion protein. The protein may have at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. |
| Peptide Name | Syncytin-1Endogenous Retrovirus Group W Member 1Env-WEnvelope Polyprotein Gpr73EnverinHerv-7q Envelope ProteinHerv-W Envelope ProteinHerv-W_7q21.2 Provirus Ancestral Env PolyproteinSyncytin Cleaved Into - Surface ProteinSuGp50 - Transmembrane ProteinTmGp24 |
| Cellular Localisation | Surface Protein: Cell MembranePeripheral Membrane ProteinThe Surface Protein Is Not Anchored To The MembraneBut Localizes To The Extracellular Surface Through Its Binding To TmTransmembrane Protein: Cell MembraneSingle-Pass Type I Membrane ProteinSyncytin-1: Virion |
| Alternative Peptide Names | Syncytin-1 proteinEndogenous Retrovirus Group W Member 1 proteinEnv-W proteinEnvelope Polyprotein Gpr73 proteinEnverin proteinHerv-7q Envelope Protein proteinHerv-W Envelope Protein proteinHerv-W_7q21.2 Provirus Ancestral Env PolyproteinSyncytin Cleaved Into - Surface Protein proteinSu proteinGp50 - Transmembrane Protein proteinTm proteinGp24 proteinERVW-1 proteinERVWE1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
