| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
STK3/MST-2 Positive Control for STJ506030 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Stk3 |
| Gene ID: |
56274 |
| Uniprot ID: |
STK3_MOUSE |
| Specificity: |
This is positive control is recommended for use in combination with STK3/MST-2 antibody STJ506030. |
| Immunogen: |
Purified Mammalian STE20-like protein kinase 2/STE20-like kinase MST2/STK3. |
| Post Translational Modifications | Phosphorylation at Thr-117 and Thr-390 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). |
| Function | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38. |
| Peptide Name | Serine/Threonine-Protein Kinase 3Mammalian Ste20-Like Protein Kinase 2Mst-2Ste20-Like Kinase Mst2 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda SubunitMst2/N - Serine/Threonine-Protein Kinase 3 20kda SubunitMst2/C |
| Database Links | Reactome: R-MMU-2028269 |
| Cellular Localisation | CytoplasmNucleusThe Caspase-Cleaved Form Cycles Between Nucleus And CytoplasmPhosphorylation At Thr-117 Leads To Inhibition Of Nuclear Translocation |
| Alternative Peptide Names | Serine/Threonine-Protein Kinase 3 proteinMammalian Ste20-Like Protein Kinase 2 proteinMst-2 proteinSte20-Like Kinase Mst2 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda Subunit proteinMst2/N - Serine/Threonine-Protein Kinase 3 20kda Subunit proteinMst2/C proteinStk3 proteinMess1 proteinMst2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
