| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SRPK1 Positive Control for STJ505943 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
SRPK1 |
| Gene ID: |
6732 |
| Uniprot ID: |
SRPK1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with SRPK1 antibody STJ505943. |
| Immunogen: |
Purified SRSF protein kinase 1. |
| Function | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation. |
| Peptide Name | Srsf Protein Kinase 1Sfrs Protein Kinase 1Serine/Arginine-Rich Protein-Specific Kinase 1Sr-Protein-Specific Kinase 1 |
| Database Links | Reactome: R-HSA-9694631 |
| Cellular Localisation | Isoform 2: CytoplasmNucleusNucleus MatrixMicrosomeShuttles Between The Nucleus And The CytoplasmInhibition Of The Hsp90 Atpase ActivityOsmotic Stress And Interaction With Hhv-1 Icp27 Protein Can Induce Its Translocation To The NucleusKat5/Tip60 Inhibits Its Nuclear TranslocationIsoform 1: CytoplasmMainly Localized In The Microsomal Fraction And The CytoplasmAnd To A Lesser Extent In The Nuclear MatrixCytoplasmNucleoplasmNucleus SpeckleChromosomePreferentially Localizes To The Promoter Of Gene Coding Regions |
| Alternative Peptide Names | Srsf Protein Kinase 1 proteinSfrs Protein Kinase 1 proteinSerine/Arginine-Rich Protein-Specific Kinase 1 proteinSr-Protein-Specific Kinase 1 proteinSRPK1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
