Sodium/Hydrogen Exchanger 3 Antibody Positve Control Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Phosphorylation at Ser-718 by CSNK2A1 regulates SLC9A3 activity through the formation of multiple signaling complexes.
Function
Plasma membrane Na(+)/H(+) antiporter. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry, playing a key role in salt and fluid absorption and pH homeostasis. Major apical Na(+)/H(+) exchanger in kidney and intestine playing an important role in renal and intestine Na(+) absorption and blood pressure regulation.
Peptide Name
Sodium/Hydrogen Exchanger 3Na(+/H(+ Exchanger 3Nhe-3Solute Carrier Family 9 Member 3
Apical Cell MembraneMulti-Pass Membrane ProteinCell MembraneRecycling Endosome MembraneEarly Endosome MembraneIn Intestinal Epithelial CellsLocalizes To The Ileal Brush BorderPhosphorylation At Ser-663 By Sgk1 Is Associated With Increased Abundance At The Cell MembraneAngiotensin-2 Enhances Apical Expression
Alternative Peptide Names
Sodium/Hydrogen Exchanger 3 proteinNa(+/H(+ Exchanger 3 proteinNhe-3 proteinSolute Carrier Family 9 Member 3 proteinSLC9A3 proteinNHE3 protein
