Serine/threonine-protein kinase 4/MST-1 Blocking Peptide for STJ506028 peptide (STJ505782)

SKU:
STJ505782-250

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Applications: Immunodepletion/Immunocompetition
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Serine/threonine-protein kinase 4/MST-1 Blocking Peptide for STJ506028 is synthetically produced from the 400-450 sequence and is suitable for use in western blot applications.
Formulation: Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: STK4
Gene ID: 6789
Uniprot ID: STK4_HUMAN
Immunogen Region: 400-450
Specificity: This blocking peptide is recommended for use in combination with Serine/threonine-protein kinase 4/MST-1 antibody, STJ506028
Immunogen: Synthetic peptide taken within amino acid region 400-450 on human Mammalian STE20-like protein kinase 1/STE20-like kinase MST1/STK4.
Tissue Specificity Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
Post Translational Modifications Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-387 by PKB/AKT1, leads to inhibition of its: kinase activity, nuclear translocation and autophosphorylation at Thr-183. It also diminishes its cleavage by caspases and its ability to phosphorylate FOXO3. Proteolytically cleaved by caspase-3 during apoptosis at Asp-326 and Asp-349 resulting in a 37 kDa or a 39 kDa subunit respectively. The 39 kDa subunit is further cleaved into the 37 kDa form. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). It is less likely that cleavage at Asp-349 is a prerequisite for activation as this site is not conserved in the murine ortholog.
Function Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
Peptide Name Serine/Threonine-Protein Kinase 4
Mammalian Ste20-Like Protein Kinase 1
Mst-1
Ste20-Like Kinase Mst1
Serine/Threonine-Protein Kinase Krs-2 Cleaved Into - Serine/Threonine-Protein Kinase 4 37kda Subunit
Mst1/N - Serine/Threonine-Protein Kinase 4 18kda Subunit
Mst1/C
Database Links Reactome: R-HSA-2028269
Cellular Localisation Cytoplasm
Nucleus
The Caspase-Cleaved Form Cycles Between The Nucleus And Cytoplasm
Alternative Peptide Names Serine/Threonine-Protein Kinase 4 protein
Mammalian Ste20-Like Protein Kinase 1 protein
Mst-1 protein
Ste20-Like Kinase Mst1 protein
Serine/Threonine-Protein Kinase Krs-2 Cleaved Into - Serine/Threonine-Protein Kinase 4 37kda Subunit protein
Mst1/N - Serine/Threonine-Protein Kinase 4 18kda Subunit protein
Mst1/C protein
STK4 protein
KRS2 protein
MST1 protein

Information sourced from Uniprot.org

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