Secondary Antibodies By Species | St John's Laboratory

Goat Anti-Rabbit IgG H&L antibody {Cy5} (STJS001045)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IHC/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG H&L antibody {Cy7} (STJS001131)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IHC/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Western blot analysis of extracts of HeLa cells, using GAPDH (STJ11103681) antibody as the primary antibody at dilution of 1:80000. Secondary antibody: using HRP Goat Anti-rabbit IgG (H+L) antibody (STJS000856) at 1:4000-1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Goat Anti-Rabbit IgG H&L antibody {HRP} (STJS000856)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/DB/IHC-P/ELISA

Reactivity :

Rabbit

£46.50 - £68.50

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Goat Anti-Rabbit IgG H&L antibody {Red} (STJS000959)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IHC/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG H&L antibody {TRITC} (STJS000916)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IHC/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Immunofluorescence analysis of HeLa cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (STJ11103694) (40x lens) , the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (STJS001206) used at dilution of 1 : 200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Goat Anti-Rabbit IgG H+L antibody {ABflo® 488} (STJS001206)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (STJ248506, dilution 1:200) followed by a further incubation with ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (STJS001186, dilution 1:400). DAPI was used for nuclear staining (Blue). Objective: 100x.

Goat Anti-Rabbit IgG H+L antibody {ABflo® 594} (STJS001186)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Goat Anti-Rabbit IgG H+L antibody {AP} (STJS001168)

Host :

Goat

Applications :

WB

Reactivity :

Rabbit

£51.50 - £79.50

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Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb (STJ235679, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:100) (Red) , ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (STJS001209, dilution 1:200) (Green)

Goat Anti-Rabbit IgG H+L antibody {Cy3} (STJS001166)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Immunofluorescence analysis of HepG2 cells, using FITC Goat Anti-Rabbit IgG (H+L) (STJ111061671) as the primary antibody at dilution of 1:100. <br/>Secondary antibody: FITC Goat Anti-Rabbit IgG (H+L) (STJS001169). Blue: DAPI for nuclear staining.

Goat Anti-Rabbit IgG H+L antibody {FITC} (STJS001169)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (STJ22487) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. <br/>Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (STJS001187) at 1:200 dilution. <br/>Blue: DAPI for nuclear staining.

Goat Anti-Rabbit IgG H+L antibody {Rhodamine} (STJS001187)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC-P/IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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To use the Alexa Fluors with fluorescent imagers, use a spectral line of the blue laser diode for Alexa Fluors 405, a cyan (488 nm) laser for Alexa Fluors 488, a yellow (526 nm) laser for Alexa Fluor 550 or 594, and a red (633 nm) laser for Alexa Fluor 649. The Alexa Fluor 680 and 790 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, and 800 nm

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000218)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000261)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Inhaled Lipid Nanoparticles Alleviate Established Pulmonary Fibrosis Small Yang Yang IF mouse murine lung epithelial-12 (MLE-12) cell

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000304)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000347)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000390)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Losartan attenuates acetic acid enema-induced visceral hypersensitivity by inhibiting the ACE1/Ang II/AT1 receptor axis in enteric glial cells EUROPEAN JOURNAL OF PHARMACOLOGY Fei Dai IF Rat colon tissue enteric glial cells (EGCs)

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000433)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 594} (STJS000476)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 633} (STJS000519)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000562)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 660} (STJS000605)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 680} (STJS000648)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC/WB

Reactivity :

Rabbit

£138.00 - £757.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 750} (STJS000691)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£160.00 - £984.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 790} (STJS000734)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£160.00 - £984.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Biotin} (STJ99670)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£82.00 - £150.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Biotin} (STJS000152)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Rabbit

£75.00 - £237.00

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Zhang, Zi-Bin, et al. "miRNA-7a-2-3p Inhibits Neuronal Apoptosis in Oxygen-Glucose Deprivation (OGD) Model. " Frontiers in neuroscience 13 (2019).

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {FITC} (STJ99668)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£82.00 - £150.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {FITC} (STJS000184)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rabbit

£75.00 - £237.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Gold} (STJ99356)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£129.00

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TBBPA causes apoptosis in grass carp hepatocytes involving destroyed ER-mitochondrial function. CHEMOSPHERE Shiwen Xu WB Fish 1:8000 L8824 cell

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {HRP} (STJ99666)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/ELISA

Reactivity :

Rabbit

£82.00 - £150.00

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Polysaccharides from Tetrastigma Hemsleyanum Diels et Gilg ameliorated inflammatory bowel disease by rebuilding the intestinal mucosal barrier and inhibiting inflammation through the SCFA-GPR41/43 signaling pathway. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Fangmei Zhou

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Unconjugated} (STJ99672)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£82.00 - £150.00

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 488} (STJS000813)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 555} (STJS000804)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 568} (STJS000822)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000832)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 633} (STJS000840)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 647} (STJS000850)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit Rabbit IgG Conjugated-F (ab ) ₂, Fc specific antibody {ABflo® 647} (STJS001256)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF-P/FC

Reactivity :

Rabbit

£71.50 - £120.50

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Western blot analysis of various lysates using Goat Anti-Mouse Anti-Rabbit IgG H (HRP polymer) (STJS000859) at 1:1000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Goat Anti-Rabbit Rabbit IgG H antibody {HRP} (STJS000859)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Rabbit

£46.50 - £68.50

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Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2. 5 Mu g/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red). The secondary antibody used was ABflo® 405-conjugated Goat Anti-Rabbit IgG (H+L) (STJS001192) at 1:100.

Goat Anti-Rabbit Rabbit IgG H+L antibody {ABflo® 405} (STJS001192)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Immunofluorescence analysis of HeLa cells, using ACO2 antibody (STJ22487) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. <br/>Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (STJS001189) at 1:200 dilution. <br/>Blue: DAPI for nuclear staining.

Goat Anti-Rabbit Rabbit IgG H+L antibody {ABflo® 488} (STJS001189)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (STJ248506, dilution 1:200) followed by a further incubation with ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L) (STJS001196, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Goat Anti-Rabbit Rabbit IgG H+L antibody {ABflo® 647} (STJS001196)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£51.50 - £79.50

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Confocal imaging of paraffin-embedded Mouse brain using Beta III-Tubulin Rabbit mAb (STJ1111133, dilution 1:200) followed by a further incubation with ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L) (STJS001205, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Goat Anti-Rabbit Rabbit IgG H+L antibody {ABflo® 750} (STJS001205)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£46.50 - £68.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10µ K-562 cells with 1 Mu g of TriMethyl-Histone H3-K4 Rabbit mAb (STJ1111286) , followed by incubation with Goat Anti-Rabbit IgG (H+L) (STJS001204). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 around RPL30 gene.

Goat Anti-Rabbit Rabbit IgG H+L antibody {Unconjugated} (STJS001204)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/ELISA/CUT&Tag

Reactivity :

Rabbit

£44.50 - £65.50

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Goat Anti-Rat IgG (Full length) antibody {DyLight®405} (STJ140234)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Immunostaining- C57BL/6J mouse retina labeled with: Green- 1st antibody Cone Arrestin (1:1,000) ; 2nd antibody goat anti-rat (STJ140235, 1:1,000) , Red- 1st antibody Rhodopsin (1:1,000) ; 2nd antibody goat anti-mouse (STJ140232, 1:1,000) , Blue- Nuclear staining (DAPI) , Scale bar = 40 um

Goat Anti-Rat IgG (Full length) antibody {DyLight®488} (STJ140235)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Immunostaining- rd16 (retinal degeneration) mouse retina labeled with: Red- 1st antibody GFAP (1/500) ; 2nd antibody goat anti-rat (STJ140236, 1:1000) , Blue- Nuclear staining (DAPI) , scale bar = 40 um

Goat Anti-Rat IgG (Full length) antibody {DyLight®550} (STJ140236)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Goat Anti-Rat IgG (Full length) antibody {DyLight®633} (STJ140237)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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