Conjugated Secondary Antibodies | St John's Laboratory

To use the Alexa Fluors with fluorescent imagers, use a spectral line of the blue laser diode for Alexa Fluors 405, a cyan (488 nm) laser for Alexa Fluors 488, a yellow (526 nm) laser for Alexa Fluor 550 or 594, and a red (633 nm) laser for Alexa Fluor 649. The Alexa Fluor 680 and 790 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, and 800 nm

Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000245)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000331)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000417)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000460)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 594} (STJS000503)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 633} (STJS000546)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000589)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 660} (STJS000632)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 680} (STJS000675)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/FC/WB

Reactivity :

Monkey

$342.24 - $1,535.94

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 750} (STJS000718)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$433.32 - $1,984.44

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 790} (STJS000761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Monkey

$433.32 - $1,984.44

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {Biotin} (STJS000175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Monkey

$164.22 - $641.70

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {FITC} (STJS000207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/FC

Reactivity :

Monkey

$164.22 - $641.70

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Mouse Anti-Monkey IgG Heavy & Light Chain antibody {HRP} (STJS000143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Monkey

$164.22 - $641.70

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Mouse Anti-Pig IgG antibody {Biotin} (STJ99432)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Pig

$342.24

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Mouse Anti-Pig IgG antibody {FITC} (STJ99482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Pig

$342.24

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Mouse Anti-Pig IgG antibody {Gold} (STJ99382)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Pig

$342.24

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Mouse Anti-Pig IgG antibody {HRP} (STJ99532)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Pig

$342.24

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000246)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000289)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000332)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 594} (STJS000504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 633} (STJS000547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000590)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 660} (STJS000633)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 680} (STJS000676)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/FC/WB

Reactivity :

Pig

$342.24 - $1,535.94

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 750} (STJS000719)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$433.32 - $1,984.44

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Alexa Fluor 790} (STJS000762)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Pig

$433.32 - $1,984.44

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {Biotin} (STJS000153)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Pig

$133.86 - $492.66

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {FITC} (STJS000185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/FC

Reactivity :

Pig

$133.86 - $492.66

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Mouse Anti-Pig IgG Heavy & Light Chain antibody {HRP} (STJS000121)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Pig

$133.86 - $492.66

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Western blot analysis of lysates from HeLa cells, using Beta-Actin (STJ113532) antibody as the primary antibody at dilution of 1:10000. <br/>Secondary antibody: using HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L) antibody (STJS001212) ) at 1:5000-1:10000 dilution.

Mouse Anti-Rabbit IgG H+L antibody {HRP} (STJS001212)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Rabbit

$93.15 - $155.25

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Dose response curve of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 1. 22*10^-4 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated thatMouse Anti-Rabbit IgG (Fc) mAb has highly specific recognition of Rabbit IgG while no or minimal cross reactivity to Rabbit IgMã€ Human IgGã€Mouse IgGã€Rat IgG.

Mouse Anti-Rabbit IgG Heavy chain (96-323 aa) antibody {HRP} (STJS001224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Rabbit

$71.07 - $109.71

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Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (STJS001197) at 1:10000 dilution. <br/>Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 100 ng per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Mouse Anti-Rabbit IgG Light Chain antibody {HRP} (STJS001197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Rabbit

$93.15 - $155.25

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Anti-Rabbit IgG antibody western blot native denatured rabbit IgG STJS001235

Mouse Anti-Rabbit Rabbit IgG antibody {HRP} (STJS001235)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ELISA

Reactivity :

Rabbit

$184.23 - $440.91

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c-Myc Tag antibody western blot CHO-K1 HRP Conjugate STJS001240

Mouse Anti-Species-independent c-Myc Tag antibody {HRP} (STJS001240)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/IHC-P/IP

Reactivity :

Species-independent

$258.75 - $1,133.67

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Flag tag antibody western blot CHO-K1 cells STJS001239

Mouse Anti-Species-independent Flag Tag antibody {HRP} (STJS001239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Species-independent

$258.75 - $1,133.67

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FLAG tag antibody western blot CHO-K1 cells STJS001241

Mouse Anti-Species-independent Flag Tag antibody {HRP} (STJS001241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Species-independent

$258.75 - $1,133.67

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GST tag antibody western blot recombinant GST fusion proteins STJS001230

Mouse Anti-Species-independent GST Tag antibody {HRP} (STJS001230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Species-independent

$258.75 - $1,133.67

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HA Tag antibody western blot CHO-K1 cells STJS001229

Mouse Anti-Species-independent HA Tag antibody {HRP} (STJS001229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Species-independent

$258.75 - $1,133.67

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HIS Tag antibody western blot recombinant protein STJS001238

Mouse Anti-Species-independent His Tag antibody {HRP} (STJS001238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Species-independent

$258.75 - $1,133.67

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Mouse Anti-Tag Biotin antibody {Biotin} (STJ16100612)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/ELISA/FC

Reactivity :

Tag

$638.25

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Rabbit Anti-BSA BSA antibody {Biotin} (STJS000075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

BSA

$284.28

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Conjugation