Conjugated Secondary Antibodies | St John's Laboratory

To use the Alexa Fluors with fluorescent imagers, use a spectral line of the blue laser diode for Alexa Fluors 405, a cyan (488 nm) laser for Alexa Fluors 488, a yellow (526 nm) laser for Alexa Fluor 550 or 594, and a red (633 nm) laser for Alexa Fluor 649. The Alexa Fluor 680 and 790 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, and 800 nm

Rabbit Anti-Human IgG+IgM+IgA antibody {Alexa Fluor 660} (STJS000618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£174.00 - £735.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {Alexa Fluor 680} (STJS000661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/FC/WB

Reactivity :

Human

£174.00 - £735.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {Alexa Fluor 750} (STJS000704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£227.00 - £1,006.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {Alexa Fluor 790} (STJS000747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£227.00 - £1,006.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {Biotin} (STJS000165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Human

£93.00 - £335.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {FITC} (STJS000197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/FC

Reactivity :

Human

£93.00 - £335.00

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Rabbit Anti-Human IgG+IgM+IgA antibody {HRP} (STJS000133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Human

£93.00 - £335.00

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Western blot analysis of various lysates, using HRP Rabbit anti-Human IgG1 (Fc) (STJS001216) at 1:4000-1:32000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Rabbit Anti-Human IgG1 Fc antibody {HRP} (STJS001216)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£67.50 - £112.50

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Dose response curve of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG2 (Fc) has highly specific recognition of Human IgG2ã€Human IgG while no or minimal cross reactivity to Human IgG1ã€Human IgG4ã€Human IgMã€Rat IgG.

Rabbit Anti-Human IgG2 Fc (99-326 aa) antibody {HRP} (STJS001227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Rabbit Anti-Human IgG4 antibody {HRP} (STJS001263)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Rabbit Anti-Human IgM antibody {Alexa Fluor 350} (STJS000232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 405} (STJS000275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 488} (STJS000318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 532} (STJS000361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 555} (STJS000404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 568} (STJS000447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 594} (STJS000490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 633} (STJS000533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 647} (STJS000576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 660} (STJS000619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 680} (STJS000662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/FC/WB

Reactivity :

Human

£248.00 - £1,113.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 750} (STJS000705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£314.00 - £1,438.00

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Rabbit Anti-Human IgM antibody {Alexa Fluor 790} (STJS000748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Human

£314.00 - £1,438.00

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Rabbit Anti-Human IgM antibody {Biotin} (STJS000166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Human

£161.00 - £680.00

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Rabbit Anti-Human IgM antibody {FITC} (STJS000198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/FC

Reactivity :

Human

£161.00 - £680.00

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Rabbit Anti-Human IgM antibody {HRP} (STJS000134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Human

£161.00 - £680.00

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Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0. 5 Mu g/mL to 3. 05Ã—10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1ã€Human IgG2ã€Human IgG3ã€Human IgG4ã€Mouse IgMã€Rat IgM.

Rabbit Anti-Human IgM antibody {HRP} (STJS001219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£46.50 - £68.50

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Rabbit Anti-Human IgM Mu chain antibody {Biotin} (STJ99426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£206.00

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Rabbit Anti-Human IgM Mu chain antibody {FITC} (STJ99476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£206.00

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Rabbit Anti-Human IgM Mu chain antibody {Gold} (STJ99376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£206.00

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Rabbit Anti-Human IgM Mu chain antibody {HRP} (STJ99526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£206.00

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Rabbit Anti-Human Kappa Light Chain antibody {HRP} (STJS001265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£67.50 - £112.50

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Rabbit Anti-Human/Mouse/Rat Light Chain antibody {Biotin} (STJ501176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Rabbit Anti-Human/Mouse/Rat Light Chain antibody {FITC} (STJ501177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of extracts of various cell lines, using HRP rabbit Anti-Camelid VHH Antibody at 1:1000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Rabbit Anti-Llama VHH antibody {HRP} (STJS000855)

Host :

Rabbit

Applications :

WB

Reactivity :

Llama

£201.50 - £379.50

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Rabbit Anti-Monkey IgE Fc antibody {HRP} (STJS001262)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Monkey

£79.50 - £136.50

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 594} (STJS000491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 633} (STJS000534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 660} (STJS000620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 680} (STJS000663)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/FC/WB

Reactivity :

Monkey

£161.00 - £680.00

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Rabbit Anti-Monkey IgG Heavy & Light Chain antibody {Alexa Fluor 750} (STJS000706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/FC

Reactivity :

Monkey

£206.00 - £896.00

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Conjugation