| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, 300mM NaCl, pH 7.4.or Supplied as a 0.22 Mu m filtered solution in PBS, 300mM NaCl, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV Spike RBD at 2 Mu g/mL (100 Mu L/well) can bind Human ACE2 with a linear range of 0.1-5.18 ng/mL. |
| Immunogen Region: | Arg306-Phe527 |
| Immunogen: | Recombinant SARS-CoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg306-Phe527) of sars-cov Spike RBD (Accession #NP_828851.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | RVVPSGDVVRFPNITNLCPF GEVFNATKFPSVYAWERKKI SNCVADYSVLYNSTFFSTFK CYGVSATKLNDLCFSNVYAD SFVVKGDDVRQIAPGQTGVI ADYNYKLPDDFMGCVLAWNT RNIDATSTGNYNYKYRYLRH GKLRPFERDISNVPFSPDGK PCTPPALNCYWPLNDYGFYT TTGIGYQPYRVVVLSFELLN APATVCGPKLSTDLIKNQCV NF |
| Background | Recombinant SARS-CoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg306-Phe527) of sars-cov Spike RBD (Accession #NP_828851.1) fused with a 6×His tag at the C-terminus. |
Information sourced from Uniprot.org