| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike RBD at 2 Mu g/mL (100 Mu L/well) can bind recombinant Human ACE2 with a linear range of 0.03-37.5 ng/mL. |
| Immunogen Region: | L452R, E484Q |
| Immunogen: | Recombinant SARS-COV-2 Spike RBD (L452R, E484Q) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541 (L452R, E484Q) ) of SARS-COV-2 RBD (Accession #YP_009724390.1) fused with a 6×His tag |
| Immunogen Sequence: | RVQPTESIVRFPNITNLCPF GEVFNATRFASVYAWNRKRI SNCVADYSVLYNSASFSTFK CYGVSPTKLNDLCFTNVYAD SFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNS NNLDSKVGGNYNYRYRLFRK SNLKPFERDISTEIYQAGST PCNGVQGFNCYFPLQSYGFQ PTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKC VNF |