Applications: | WB |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | ROCK1 Positive Control for STJ502819 is synthetically produced from the sequence and is suitable for use in western blot applications. |
Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
Dilution Range: | WB: 1:500 IHC: 1:100 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Gene Symbol: | ROCK1 |
Gene ID: | 6093 |
Uniprot ID: | ROCK1_HUMAN |
Specificity: | This is positive control is recommended for use in combination with ROCK1 antibody STJ502819. |
Tissue Specificity | Detected in blood platelets. |
Post Translational Modifications | Autophosphorylated on serine and threonine residues. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
Function | Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. |
Peptide Name | Rho-Associated Protein Kinase 1 Renal Carcinoma Antigen Ny-Ren-35 Rho-Associated - Coiled-Coil-Containing Protein Kinase 1 Rho-Associated - Coiled-Coil-Containing Protein Kinase I Rock-I P160 Rock-1 P160rock |
Database Links | Reactome: R-HSA-111465 Reactome: R-HSA-3928662 Reactome: R-HSA-3928663 Reactome: R-HSA-416482 Reactome: R-HSA-416572 Reactome: R-HSA-4420097 Reactome: R-HSA-5627117 Reactome: R-HSA-6798695 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013407 Reactome: R-HSA-9013422 Reactome: R-HSA-9679191 Reactome: R-HSA-9696264 |
Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Centriole Golgi Apparatus Membrane Peripheral Membrane Protein Cell Projection Bleb Cell Membrane Lamellipodium Ruffle A Small Proportion Is Associated With Golgi Membranes Associated With The Mother Centriole And An Intercentriolar Linker Colocalizes With Itgb1bp1 And Itgb1 At The Cell Membrane Predominantly In Lamellipodia And Membrane Ruffles But Also In Retraction Fibers Localizes At The Cell Membrane In An Itgb1bp1-Dependent Manner |
Alternative Peptide Names | Rho-Associated Protein Kinase 1 protein Renal Carcinoma Antigen Ny-Ren-35 protein Rho-Associated - Coiled-Coil-Containing Protein Kinase 1 protein Rho-Associated - Coiled-Coil-Containing Protein Kinase I protein Rock-I protein P160 Rock-1 protein P160rock protein ROCK1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance