| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
ROCK1 Positive Control for STJ502819 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500IHC: 1:100 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
ROCK1 |
| Gene ID: |
6093 |
| Uniprot ID: |
ROCK1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with ROCK1 antibody STJ502819. |
| Tissue Specificity | Detected in blood platelets. |
| Post Translational Modifications | Autophosphorylated on serine and threonine residues. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
| Function | Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. |
| Peptide Name | Rho-Associated Protein Kinase 1Renal Carcinoma Antigen Ny-Ren-35Rho-Associated - Coiled-Coil-Containing Protein Kinase 1Rho-Associated - Coiled-Coil-Containing Protein Kinase IRock-IP160 Rock-1P160rock |
| Database Links | Reactome: R-HSA-111465Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-416482Reactome: R-HSA-416572Reactome: R-HSA-4420097Reactome: R-HSA-5627117Reactome: R-HSA-6798695Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013407Reactome: R-HSA-9013422Reactome: R-HSA-9679191Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleGolgi Apparatus MembranePeripheral Membrane ProteinCell ProjectionBlebCell MembraneLamellipodiumRuffleA Small Proportion Is Associated With Golgi MembranesAssociated With The Mother Centriole And An Intercentriolar LinkerColocalizes With Itgb1bp1 And Itgb1 At The Cell Membrane Predominantly In Lamellipodia And Membrane RufflesBut Also In Retraction FibersLocalizes At The Cell Membrane In An Itgb1bp1-Dependent Manner |
| Alternative Peptide Names | Rho-Associated Protein Kinase 1 proteinRenal Carcinoma Antigen Ny-Ren-35 proteinRho-Associated - Coiled-Coil-Containing Protein Kinase 1 proteinRho-Associated - Coiled-Coil-Containing Protein Kinase I proteinRock-I proteinP160 Rock-1 proteinP160rock proteinROCK1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
