RIPK5 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Predominantly expressed in skeletal muscle and testis. Expressed in basolateral and apical membranes of all tubular epithelia. Expressed in thin ascending limb of the loop of Henle and the distal convoluted tubule. Expressed in all layers of transitional ureteric epithelium and in the ureteric smooth-muscle cells. Weakly expressed in heart, brain, placenta, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine, white blood cells, stomach, spinal cord and adrenal gland. Is widely distributed in the CNS. Also detected in several tumor cell lines. Expressed in the skin.
Function
Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. Involved in the regulation of both caspase-dependent apoptosis and caspase-independent cell death. In the skin, it plays a predominant role in suppressing caspase-dependent apoptosis in response to UV stress in a range of dermal cell types.
Peptide Name
Dual Serine/Threonine And Tyrosine Protein KinaseDusty Protein KinaseDusty PkRip-Homologous KinaseReceptor-Interacting Serine/Threonine-Protein Kinase 5Sugen Kinase 496Sgk496
Cellular Localisation
CytoplasmCell MembraneApical Cell MembraneBasolateral Cell MembraneCell JunctionDetected At Apical Cell-Cell JunctionsColocalized With Fgf Receptors To The Cell MembraneDetected In Basolateral And Apical Membranes Of All Tubular Epithelia
Alternative Peptide Names
Dual Serine/Threonine And Tyrosine Protein Kinase proteinDusty Protein Kinase proteinDusty Pk proteinRip-Homologous Kinase proteinReceptor-Interacting Serine/Threonine-Protein Kinase 5 proteinSugen Kinase 496 proteinSgk496 proteinDSTYK proteinKIAA0472 proteinRIP5 proteinRIPK5 proteinSGK496 proteinHDCMD38P protein
