Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (STJ11104084) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-XIAP antibody (1-200) [S4084RM] (STJ11104084)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of extracts of various cell lines, using XIAP antibody (STJ28949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-XIAP antibody (198-497) (STJ28949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various cells using XIAP Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-XIAP antibody (53-102 aa) (STJ96278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-XIAP antibody [PT0653R] (STJA0039287)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) 293T cells, using [KO Validated] XIAP Rabbit monoclonal antibody (STJ11104809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-XIAP antibody [S4809RM] (STJ11104809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates using 14-3-3 alpha/beta Rabbit mAb (STJ11102440) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-YWHAB antibody (147-246) [S0MR] (STJ11102440)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of L929 cells using 14-3-3 alpha/beta Rabbit polyclonal antibody (STJ26141) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YWHAB antibody (150-246) (STJ26141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using 14-3-3 Beta Polyclonal Antibody

Anti-YWHAB antibody (41-90 aa) (STJ91367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon Rabbit monoclonal antibody (STJ11102200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YWHAE antibody (1-100) [S0MR] (STJ11102200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various cells using 14-3-3 Epsilon Polyclonal Antibody diluted at 1：1000

Anti-YWHAE antibody (206-255 aa) (STJ91370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using 14-3-3 epsilon antibody (STJ26142) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YWHAE antibody (73-163) (STJ26142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and 14-3-3 gamma knockout (KO) 293T cells, using [KO Validated] 14-3-3 gamma Rabbit polyclonal antibody (STJ11100033) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-YWHAG antibody (100-200) (STJ11100033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of 14-3-3 gamma in paraffin-embedded Rat spleen using 14-3-3 gamma Rabbit polyclonal antibody (STJ26143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-YWHAG antibody (100-200) (STJ26143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using 14-3-3 gamma Rabbit mAb (STJ11102442) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-YWHAG antibody (100-200) [S2MR] (STJ11102442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of COS7 cells, using 14-3-3 gamma Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-YWHAG antibody (51-100 aa) (STJ91369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using YWHAH Rabbit pAb (STJ116342) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-YWHAH antibody (1-246) (STJ116342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-YWHAH antibody (51-100 aa) (STJ91374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Ma, Zhan, Shuping Lou, and Zheng Jiang. "PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway. " Aging (Albany NY) 12. 9 (2020) : 7985.

Anti-YWHAH antibody (STJ196152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using 14-3-3 Theta Rabbit mAb (STJ11102399) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-YWHAQ antibody (1-100) [S9MR] (STJ11102399)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various cells using 14-3-3 Theta/Tau Polyclonal Antibody diluted at 1：500

Anti-YWHAQ antibody (196-245 aa) (STJ91376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using 14-3-3 Theta Polyclonal Antibody diluted at 1：1000

Anti-YWHAQ antibody (41-90 aa) (STJ91375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using 14-3-3 Theta antibody (STJ26144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-YWHAQ antibody (49-149) (STJ26144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using YWHAZ antibody (STJ115333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-YWHAZ antibody (146-245) (STJ115333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of Rat brain using YWHAZ Rabbit polyclonal antibody (STJ29954) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YWHAZ antibody (146-245) (STJ29954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-YWHAZ antibody (196-245 aa) (STJ91373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using 14-3-3 Zeta Polyclonal Antibody diluted at 1：1000

Anti-YWHAZ antibody (24-73 aa) (STJ91372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ZO1 antibody [PT0639R] (STJA0039325)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-ZO1 tight junction protein antibody [SM0454] (STJA0010454)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Bcl-XL Blocking Peptide peptide (STJ505386)

Applications :

Immunodepletion/Immunocompetition

$380.03

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Bcl-XL Positive Control peptide (STJ505387)

Applications :

WB

$447.53

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beta Catenin Blocking Peptide peptide (STJ503875)

Applications :

Immunodepletion/Immunocompetition

$380.03

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beta Catenin Blocking Peptide peptide (STJ503876)

Applications :

Immunodepletion/Immunocompetition

$380.03

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beta Catenin Positive Control peptide (STJ503924)

Applications :

WB

$447.53

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CDKN2A/p14ARF Blocking Peptide peptide (STJ504503)

Applications :

Immunodepletion/Immunocompetition

$380.03

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CDKN2A/p14ARF Blocking Peptide peptide (STJ504504)

Applications :

Immunodepletion/Immunocompetition

$380.03

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CDKN2A/p14ARF Blocking Peptide peptide (STJ504505)

Applications :

Immunodepletion/Immunocompetition

$380.03

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CDKN2A/p14ARF Positive Control peptide (STJ504545)

Applications :

WB

$447.53

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DAP Kinase 1 Blocking Peptide peptide (STJ505694)

Applications :

Immunodepletion/Immunocompetition

$380.03

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DAP Kinase 1 Positive Control peptide (STJ505695)

Applications :

WB

$447.53

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DCC Blocking Peptide for STJ500740 peptide (STJ503948)

Applications :

Immunodepletion/Immunocompetition

$380.03

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DCC Positive Control for STJ500739 peptide (STJ503971)

Applications :

WB

$447.53

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E2F1 Blocking Peptide for STJ500810 peptide (STJ503984)

Applications :

Immunodepletion/Immunocompetition

$380.03

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E2F1 Blocking Peptide for STJ500813 peptide (STJ503986)

Applications :

Immunodepletion/Immunocompetition

$380.03

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E2F1 Blocking Peptide for STJ500815 peptide (STJ503985)

Applications :

Immunodepletion/Immunocompetition

$380.03

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E2F1 Positive Control for STJ500811 peptide (STJ504028)

Applications :

WB

$447.53

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FAK1 Blocking Peptide peptide (STJ504061)

Applications :

Immunodepletion/Immunocompetition

$380.03

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FAK1 Positive Control peptide (STJ504093)

Applications :

WB

$447.53

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Granzyme B Blocking Peptide peptide (STJ504164)

Applications :

Immunodepletion/Immunocompetition

$380.03

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