Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAS2 antibody (STJ23749) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GAS2 antibody (1-100) (STJ23749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from Rat liver, using GAS2 Rabbit monoclonal antibody (STJ11105146) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GAS2 antibody (1-100) [S5146RM] (STJ11105146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GAS2 antibody (STJ190841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Human tonsil stained with anti-Granzyme B antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic granular staining of cytotoxic T-cells.

Anti-Granzyme B antibody (73-187aa) [ZM66] (STJ180310)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$218.03 - $741.83

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Anti-Granzyme B antibody (STJ16101330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$882.23 - $1,589.63

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Anti-Granzyme B antibody [11F1] (STJ16101744)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,184.63

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Anti-Granzyme B antibody [GrB-7] (STJ16100752)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/ELISA/IHC-P

Reactivity :

Human

$717.53

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Anti-Granzyme B antibody [SM0403] (STJA0010403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Anti-Granzyme B antibody [SM0404] (STJA0010404)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

$230.18 - $315.23

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Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of lysates from THP-1 cells, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11105919) at 1:400 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11105919)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11106595) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$357.08

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Anti-GSDME antibody (1-100) (STJA0010252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of DFNA5/GSDME in U251MG, U2OS, HepG2, Hela lysates using GSDME antibody.

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$332.78

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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GSDME antibody (200-280 aa) (STJ96411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using GSDME Rabbit pAb (STJ29568) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of Gelsolin in paraffin-embedded human tonsil using Gelsolin Rabbit polyclonal antibody (STJ11104659) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (28-782) (STJ11104659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSN antibody (282-438) (STJ11101188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded mouse brain using Gelsolin Rabbit monoclonal antibody (STJ11101830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (400-500) [S0MR] (STJ11101830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-GSN antibody (STJ192165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry of rat spleen with Anti-GZMB primary antibody (STJ501289) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GZMB antibody (1-50) (STJ501289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

$577.13

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Anti-GZMB antibody (1-50) {Biotin} (STJ501291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

$713.48

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Anti-GZMB antibody (1-50) {FITC} (STJ501292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

$713.48

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Anti-GZMB antibody (100-200 aa) [PT0564] (STJ196705)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$234.90 - $340.20

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Immunofluorescence analysis of mouse spleen cells using Granzyme B Rabbit polyclonal antibody (STJ26211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GZMB antibody (100-200) (STJ26211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of baboon spleen with Anti-GZMB primary antibody (STJ501290) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-GZMB antibody (50-100) (STJ501290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

$577.13

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Anti-GZMB antibody (50-100) {Biotin} (STJ501293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

$713.48

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Anti-GZMB antibody (50-100) {FITC} (STJ501294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

$713.48

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Western blot analysis of mouse-lung lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-GZMB antibody (81-130 aa) (STJ99635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Histone 1.0 Polyclonal Antibody diluted at 1：2000

Anti-H1-0 antibody (71-120 aa) (STJ93509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H1-1 antibody (49-99 aa) (STJ195717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using HIST1H1C Rabbit polyclonal antibody (STJ11105680) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-H1-2 antibody (100-200) (STJ11105680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HIST1H1D antibody (STJA0004374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

$189.68 - $560.93

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Immunohistochemistry analysis of Histone H1. 0 in paraffin-embedded human thyroid cancer using Histone H1. 0 Rabbit monoclonal antibody (STJ11102045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-Histone H1 antibody (1-100) [S5MR] (STJ11102045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Other

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H1 antibody (1-105) (STJ23903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Histone H1 antibody [AE-4] (STJ16100269)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human/Mouse/Rat

$388.13 - $1,788.08

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Confocal imaging of U-2 OS cells using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-Histone H1.2 antibody (1-100) [S2MR] (STJ11102702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $775.58

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Immunocytochemistry analysis of Histone H1. 3 (green) in Hela using Histone H1. 3 antibody, and DAPI (blue)

Anti-Histone H1.3 antibody (5-17 aa) [R09-8H7] (STJA0011419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot analysis of extracts of various cell lines, using Histone H1. 4 antibody (STJ11103374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H1.4 antibody (1-100) (STJ11103374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1 Rabbit polyclonal antibody (STJ11105655) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGB1 antibody (1-215) (STJ11105655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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A431 whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1 (PTR2339) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431Predicted band size: 24kDaObserved band size: 24kDa

Anti-HMGB1 antibody (100-200) [PTR2339] (STJA0006264)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (STJ11101678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMGB1 antibody (100-200) [S8MR] (STJ11101678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options