Haemostasis | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 350} (STJA0006205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 405} (STJA0006206)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 488} (STJA0006207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 555} (STJA0006208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 594} (STJA0006209)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 647} (STJA0006210)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 680} (STJA0006211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {Biotin} (STJA0006212)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {Cy3} (STJA0006213)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {Cy5} (STJA0006214)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {FITC} (STJA0006215)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Anti-TUBB3 antibody [5G3] {HRP} (STJA0006216)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£144.00 - £240.00

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Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TUBB3 antibody [M7] (STJ97037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Kidney tissue, 3) Rat Heart tissue, diluted at 1:5000.

Anti-TUBB3 antibody [Mix] (STJ97041)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Brain, 3) Rat Brain, diluted at 1:5000.

Anti-TUBB3 antibody [Mix] (STJ97067)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-TUBB4A antibody (218-268 aa) (STJ192957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunocytochemistry analysis of beta IV Tubulin (green) in Hela using beta IV Tubulin antibody, and DAPI (blue)

Anti-TUBB4A antibody (430-444 aa) [R06-6A5] (STJA0011034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Immunofluorescence analysis of U2OS cells using TUBB8 antibody (STJ11102746) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TUBB8 antibody (344-444 aa) (STJ11102746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using TUBB8 Rabbit pAb (STJ11105740) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TUBB8 antibody (350-444) (STJ11105740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-Tubulin Alpha antibody [TU-02] (STJ16100478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC

Reactivity :

Human/Mouse/Pig/PlantS./Rat

£307.50 - £1,449.50

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Immunofluorescence analysis of U-2 OS cells using Tubulin beta-1 chain Rabbit monoclonal antibody (STJ11103147) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Tubulin beta-1 chain antibody (352-451) [S7MR] (STJ11103147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Dog/Monkey

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-VAV1 antibody (110-190 aa) (STJ96227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from NIH-3T3 cells, using VAV1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-VAV1 antibody (131-180 aa) (STJ96406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Vav1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VAV1 antibody (141-190 aa) (STJ96228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Rat thymus, using VAV1 antibody (STJ117302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-VAV1 antibody (374-590) (STJ117302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human appendix using VAV2 Rabbit monoclonal antibody (STJ11101896) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-VAV2 antibody (100-200) [S6MR] (STJ11101896)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various cells using Vav2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VAV2 antibody (108-157 aa) (STJ96229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit polyclonal antibody (STJ11100759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VAV2 antibody (570-700) (STJ11100759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using VAV2 Rabbit pAb (STJ113647) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-VAV2 antibody (570-700) (STJ113647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-VAV2 antibody (STJA0005638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Western blot analysis of 293T-UV cells using Vav3 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000

Anti-VAV3 antibody (139-188 aa) (STJ96230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using VAV3 Rabbit polyclonal antibody (STJ117580) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VAV3 antibody (560-680) (STJ117580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using VAV3 Rabbit mAb (STJ11103668) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-VAV3 antibody (700-800) [S8MR] (STJ11103668)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-VAV3 antibody (STJA0005639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-VCL antibody (100-200) [PT0372] (STJA0006223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Vinculin antibody (STJ116126) at dilution of 1:150. Blue: DAPI for nuclear staining.

Anti-VCL antibody (751-850) (STJ116126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Vinculin antibody (STJ26077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VCL antibody (767-1066) (STJ26077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VCL antibody (786-835 aa) (STJ96247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of HeLa cells using Vinculin Rabbit monoclonal antibody (STJ11101832, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-VCL antibody (800-900) [S2MR] (STJ11101832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Mouse brain, using Vinculin antibody (STJ11104807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-VCL antibody (800-900) [S4807RM] (STJ11104807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-VEGF antibody [VEGF-21] (STJ16101009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human/Dog/Mouse/Rabbit/Rat

£287.50 - £1,324.50

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Western blot analysis of extracts of various cell lines, using VEGF-D antibody (STJ11102974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VEGF-D antibody (200-300) [S4MR] (STJ11102974)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit polyclonal antibody (STJ27525) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (100-200) (STJ27525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-VEGF-A (PT0865) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LnCapPredicted band size: 27kDaObserved band size: 42kDa

Anti-VEGFA antibody (100-200) [PT0865] (STJA0006227)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of mouse-kidney tissue. 1, VEGF-A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-VEGFA antibody (110-159 aa) (STJ96235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig/Rabbit

£48.00 - £252.00

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Western blot analysis of various lysates, using VEGFA Mouse monoclonal antibody (STJ119887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VEGFA antibody (207-327) [S7MM] (STJ119887)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit polyclonal antibody (STJ114190) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (27-126) (STJ114190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 using VEGF antibody (STJ11104459) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (51-327) (STJ11104459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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