Circadian Clock | Research Area | St John's Laboratory

Western blot analysis of SK-BR-3, using DBP Rabbit polyclonal antibody (STJ117465) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-DBP antibody (1-325) (STJ117465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-DBP antibody (STJA0007288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-EP300 antibody (1-270) (STJ114983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of EP300 in paraffin-embedded mouse spleen tissue using EP300 Rabbit polyclonal antibody (STJ11105314) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EP300 antibody (940-1030) (STJ11105314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, p300 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EP300 antibody (Internal) (STJ97645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using p300 monoclonal antibody against truncated p300-His recombinant protein (1).

Anti-EP300 antibody [7D8A6] (STJ98299)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using F7 Rabbit polyclonal antibody (STJ118484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-F7 antibody (240-400) (STJ118484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using FBXL3 Rabbit pAb (STJ11106134) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-FBXL3 antibody (1-110 aa) (STJ11106134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis using HDAC3 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , C6 (3) and COS (4) cell lysate.

Anti-HDAC3 antibody (224-428 aa) [7G6C5] (STJ98129)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-HDAC3 antibody (299-428) (STJ23930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (STJ114416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HDAC3 antibody (329-428) (STJ114416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HDAC3 Rabbit polyclonal antibody (STJ118890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC3 antibody (329-428) (STJ118890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g HDAC3 Rabbit monoclonal antibody (STJ11101684). Western blot was performed from the immunoprecipitate using HDAC3 Rabbit monoclonal antibody (STJ11101684) at a dilution of 1:1000.

Anti-HDAC3 antibody (329-428) [S4MR] (STJ11101684)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using HDAC3 Polyclonal Antibody diluted at 1：1000

Anti-HDAC3 antibody (379-428 aa) (STJ93479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using SMAP45 Polyclonal Antibody diluted at 1：2000

Anti-HDAC3 antibody (379-428 aa) (STJ95704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of HDAC3 in (1) HeLa lysates; (2) NIH/3T3 lysates using HDAC3 antibody.

Anti-HDAC3 antibody (400-428 aa) [R07-8E9] (STJA0014653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis using HDAC3 monoclonal antibody against THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3, HeLa cell lysate.

Anti-HDAC3 antibody (STJ98496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Rabbit

£144.00 - £348.00

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Western blot detection of HDAC3 in THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.

Anti-HDAC3 antibody [3G3-H6-H10] (STJ99157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of PRIC285 Antibody. The lane on the right is blocked with the PRIC285 peptide.

Anti-HELZ2 antibody (1909-1958 aa) (STJ95222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 + 2 primary antibody (STJ501335) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) (STJ501335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {Biotin} (STJ501338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {FITC} (STJ501339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 primary antibody (STJ501334) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 antibody (790-826) (STJ501334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {Biotin} (STJ501336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {FITC} (STJ501337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF1 Alpha antibody (552-826 aa) (STJ11106258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) Hela, 2) 293, 3)mouse braincells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-HIF1A antibody (328-377 aa) (STJ93498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HIF1a antibody at 2500 dilution; HT1080 cells in the presence of CoCl2; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-HIF1A antibody (470 to 590aa) (STJ140124)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Western blot analysis of lysates from HepG2 cells, using HIF1 Alpha Rabbit polyclonal antibody (STJ29997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIF1A antibody (600-826) (STJ29997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells, using HIF1 Alpha Rabbit pAb (STJ113863) at 1:1000 dilution. HeLa cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIF1A antibody (670-826) (STJ113863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HIF1A antibody (780aa C-Term) (STJ140040)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis using HIF-1 Alpha monoclonal antibody against Cos7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.

Anti-HIF1A antibody [1A3] (STJ98135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis of 1) HepG2 Cell, 2) HepG2 Treated with CoCl2 cell lysate using HIF-1α mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HIF1A antibody [Mix] (STJ196669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-Hydroxy-HIF1A-P564 antibody (STJ11106685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using KAT13A/ SRC1 Rabbit mAb (STJ11103645) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-KAT13A/SRC1 antibody (400-750) [S5MR] (STJ11103645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of A549 cells using KLF15 antibody (STJ115381). Blue: DAPI for nuclear staining.

Anti-KLF15 antibody (1-260) (STJ115381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with KLF15 using KLF15 Rabbit polyclonal antibody (STJ29274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KLF15 antibody (1-260) (STJ29274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis using KLF15 monoclonal antibody against HepG2 (1) and SMMC-7721 (2) cell lysate.

Anti-KLF15 antibody [2G8] (STJ98198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of MCF7 cells, using MED1 antibody (STJ24540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MED1 antibody (1-300) (STJ24540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (1423-1472 aa) (STJ96090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (641-690 aa) (STJ96089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MED1 (PTR1203) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: DaudiPredicted band size: 220kDaObserved band size: 220kDa

Anti-MED1 antibody (650-750) [PTR1203] (STJA0006273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemistry analysis of MEF2C in paraffin-embedded human breast cancer using MEF2C Rabbit polyclonal antibody (STJ24543) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MEF2C antibody (170-380) (STJ24543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse brain using MEF2C Rabbit polyclonal antibody (STJ114263) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MEF2C antibody (170-473) (STJ114263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293T cells using MEF-2C Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MEF2C antibody (362-411 aa) (STJ94067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MEF2D Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MEF2D antibody (410-459 aa) (STJ94069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using MEF2D Rabbit polyclonal antibody (STJ118826) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-MEF2D antibody (431-521) (STJ118826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using PBEF/Visfatin/NAMPT Rabbit polyclonal antibody (STJ24679). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-NAMPT antibody (181-382) (STJ24679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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