Autophagy | Research Area | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using DYNLL1 Rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DYNLL1 antibody (1-89) (STJ116707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using DYNLL1 antibody (STJ28309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-DYNLL1 antibody (1-89) (STJ28309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human colon using DYNLL1 Rabbit monoclonal antibody (STJ11102053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DYNLL1 antibody (1-89) [S3MR] (STJ11102053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval.

Anti-DYNLL2 antibody (1-50 aa) (STJ92797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using DYNLL2 Rabbit polyclonal antibody (STJ115827) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DYNLL2 antibody (1-89) (STJ115827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using DYNLL2 Rabbit polyclonal antibody (STJ115828) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DYNLL2 antibody (1-89) (STJ115828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit polyclonal antibody (STJ23475) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EEF1A1 antibody (123-462) (STJ23475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of C6 cells using eEF1A1 Rabbit monoclonal antibody (STJ11101355, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-EEF1A1 antibody (250-350) [S5MR] (STJ11101355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (STJ119870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EEF1A1 antibody (301-400) (STJ119870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Elongation Factor 1A1 (green) in Hela using Elongation Factor 1A1 antibody, and DAPI (blue).

Anti-EEF1A1 antibody (325-338 aa) [R04-3H4] (STJA0012354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using eEF1A1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EEF1A1 antibody (434-447 aa) [R05-4K5] (STJA0011256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of various lysates using EPAS1/HIF2 Alpha Rabbit pAb (STJ29691) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EPAS1 antibody (678-745) (STJ29691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EPAS1 antibody (STJ96690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of FUNDC1 in paraffin-embedded Mouse brain tissue using FUNDC1 Rabbit polyclonal antibody (STJ118748) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FUNDC1 antibody (1-155) (STJ118748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of AD293 cells using FUNDC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-FUNDC1 antibody (1-50 aa) (STJ97353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of K-562 cells, using FUNDC1 antibody (STJ11104646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FUNDC1 antibody (50-155) (STJ11104646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using GABARAP antibody (STJ114442) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GABARAP antibody (1-100) (STJ114442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using GABARAP antibody (STJ115794).

Anti-GABARAP antibody (1-100) (STJ115794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using GABARAP Rabbit monoclonal antibody (STJ11102043) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GABARAP antibody (1-100) [S3MR] (STJ11102043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF-7 cells using GABARAP antibody (STJ27583). Blue: DAPI for nuclear staining.

Anti-GABARAP antibody (1-117) (STJ27583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GABARAP antibody (8-58 aa) (STJ194630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-GABARAP antibody (N-Term) (STJ13101124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-GABARAP antibody (N-Term) (STJ13101168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-GABARAP antibody (N-Term) (STJA0005686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-GABARAP antibody (STJ13101123)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of various lysates using GABARAPL1 Rabbit mAb (STJ11107315) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-GABARAPL1 antibody (1-100 aa) [SRM] (STJ11107315)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of Mouse brain, using GABARAPL1 antibody (STJ110100) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-GABARAPL1 antibody (1-50) (STJ110100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GABARAPL1 antibody (32-82) (STJA0006650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse heart using GABARAPL2 antibody (STJ110092) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GABARAPL2 antibody (1-117) (STJ110092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using GABARAPL2 Rabbit polyclonal antibody (STJ117055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GABARAPL2 antibody (1-50) (STJ117055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-GABARAPL2 antibody (26-75 aa) (STJ98595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using GABARAPL2 Rabbit mAb (STJ11102498) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-GABARAPL2 antibody (38-117) [S8MR] (STJ11102498)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GFAP antibody (STJ11101596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GFAP antibody (1-100) [S6MR] (STJ11101596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GFAP antibody (1-75) (STJ23770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes

Anti-GFAP antibody (150-250aa) [ZR356] (STJ180514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £572.50

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Anti-GFAP antibody (250-300 aa) (STJ13100497)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£331.50

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Anti-GFAP antibody (300-432 aa) [ABT-GFAP] (STJ196765)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GFAP (ABT176) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 50kDaObserved band size: 57kDa

Anti-GFAP antibody (300-432 aa) [ABT176] (STJ197157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT331) Antibody

Anti-GFAP antibody (300-432 aa) [ABT331] (STJ197231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT470) Antibody

Anti-GFAP antibody (300-432 aa) [ABT470] (STJ190068)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-GFAP antibody (300-432 aa) [ABT484] (STJ197230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human cerebrum tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT518) Antibody

Anti-GFAP antibody (300-432 aa) [PT1995] (STJ196968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Fluorescence multiplex immunohistochemical analysis of mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit. GFAP mouse monoclonal antibody (STJA0006242 green), S100 mouse monoclonal antibody (STJ197266 cyan), Neurofilament mouse monoclonal antibody (purple), Iba 1 mouse monoclonal antibody (red), CD8 a mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-GFAP antibody (300-432) [PTR2201/470] (STJA0006242)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GFAP antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFAP antibody (375aa C-Term) (STJ140132)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey

£135.50 - £394.50

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Immunohistochemistry of mouse brain with Anti-GFAP primary antibody (STJ501145) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GFAP antibody (50-100) (STJ501145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-GFAP antibody (50-100) {Biotin} (STJ501146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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