Rat VEGFA protein (Recombinant-Active) (No tag) (STJP011892)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenRattus norvegicus (Rat)
General Information
| Short Description | Recombinant-Active-Rat VEGFA-No tag protein was developed from mammalian cells and has a target region of No tag. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.75‑3.75 ng/mL. |
Target Information
| Gene Symbol | Vegfa |
| Gene ID | 83785 |
| Uniprot ID | VEGFA_RAT |
| Immunogen | Rattus norvegicus (Rat) |
| Immunogen Region | Ala27-Arg190 (Isoform 2) |
Additional Info
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. |
| Protein Name | Vascular Endothelial Growth Factor AVegf-AVascular Permeability FactorVpf |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-194313Reactome: -RNO-195399Reactome: -RNO-4420097Reactome: -RNO-5218921 |
| Cellular Localisation | SecretedVegf-A120 Is Acidic And Freely SecretedVegf-A164 Is More BasicHas Heparin-Binding Properties AndAlthough A Significant Proportion Remains Cell-AssociatedMost Is Freely SecretedVegf-A188 Is Very BasicIt Is Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Protein Names | Vascular Endothelial Growth Factor A proteinVegf-A proteinVascular Permeability Factor proteinVpf proteinVegfa proteinVegf protein |
Information sourced from Uniprot.org