Rat Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA Kit (STJE0006430)

Name: Rat Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA Kit (STJE0006430)
SKU: STJE0006430
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0006430

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General Information

Short Description	This Uchl1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	Uchl1
Gene ID	29545
Uniprot ID	UCHL1_RAT
Specificity	This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Function	Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Protein Name	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 Uch-L1 Neuron Cytoplasmic Protein 9.5 Pgp 9.5 Pgp9.5 Ubiquitin Thioesterase L1
Database Links	Reactome: R-RNO-5689603
Cellular Localisation	Cytoplasm Endoplasmic Reticulum Membrane Lipid-Anchor
Alternative ELISA Names	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 ELISA kit Uch-L1 ELISA kit Neuron Cytoplasmic Protein 9.5 ELISA kit Pgp 9.5 ELISA kit Pgp9.5 ELISA kit Ubiquitin Thioesterase L1 ELISA kit Uchl1 ELISA kit
Specificity	This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-109	100
EDTA plasma (n=8)	93-108	100
Cell culture media (n=8)	84-100	91

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	200.02	430.69	1489.86	208.52	445.85	1600.11
Standard deviation	11.66	25.63	51.55	14.39	21.31	64.64
CV (%)	5.83	5.95	3.46	6.90	4.78	4.04

Citations

View product citations for antibody STJE0006430 on CiteAb