Rat TNF alpha (TNF alpha) Antibody Pair (STJAP00753)
General Information
| Short Description | This TNF alpha antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R02-4W7/R01-4I9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Tnf |
| Gene ID | 24835 |
| Uniprot ID | TNFA_RAT |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated.glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor, soluble form: The soluble form is demyristoylated by SIRT6, promoting its secretion. |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Protein Name | Tumor Necrosis FactorCachectinTnf-AlphaTumor Necrosis Factor Ligand Superfamily Member 2Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane FormN-Terminal FragmentNtf - Intracellular Domain 1Icd1 - Intracellular Domain 2Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form |
| Database Links | Reactome: R-RNO-5357786Reactome: -RNO-5357905Reactome: -RNO-5357956Reactome: -RNO-5626978Reactome: -RNO-5668541Reactome: -RNO-75893 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinTumor Necrosis FactorMembrane Form: MembraneSoluble Form: SecretedC-Domain 1: SecretedC-Domain 2: Secreted |
| Alternative Antibody Pair Names | Tumor Necrosis Factor antibody pairCachectin antibody pairTnf-Alpha antibody pairTumor Necrosis Factor Ligand Superfamily Member 2 antibody pairTnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form antibody pairN-Terminal Fragment antibody pairNtf - Intracellular Domain 1 antibody pairIcd1 - Intracellular Domain 2 antibody pairIcd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form antibody pairTnf antibody pairTnfa antibody pairTnfsf2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat TNF alpha (TNF alpha) Antibody Pair (STJAP00753)
This antibody pair is designed to detect rat TNF alpha (UniProt P16599) and has confirmed reactivity with rat samples only. It is intended for in vitro research use only.
Both the capture and detection antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs at 1 mg/mL, formulated in 0.01M PBS at pH 7.2.
This pair has been validated for use in ELISA. The working dilutions for each antibody should be determined by the end user for their specific experimental conditions.
The two antibodies in this pair are identified by clone IDs R02-4W7 and R01-4I9, both raised in rabbit as monoclonal antibodies against a recombinant protein immunogen.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.