Rat TGFB2/TGF-beta-2 protein (Recombinant-Active) (Tag free) (STJP012897)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenRattus norvegicus (Rat)
General Information
| Short Description | Recombinant-Active-Rat TGFB2/TGF-beta-2-Tag free protein was developed from mammalian cells and has a target region of Tag free. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.2 Mu m filtered solution in 4 mM HCl. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | Tgfb2 |
| Gene ID | 81809 |
| Uniprot ID | TGFB2_RAT |
| Immunogen | Rattus norvegicus (Rat) |
| Immunogen Region | Ala331-Ser442 |
Additional Info
| Post Translational Modifications | Transforming growth factor beta-2: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive. |
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-2129379Reactome: -RNO-2173789Reactome: -RNO-9839389 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative Protein Names | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated Peptide proteinLap - Transforming Growth Factor Beta-2 proteinTgf-Beta-2 proteinTgfb2 protein |
Information sourced from Uniprot.org