Protein Name | Tissue FactorTfCoagulation Factor IiiCd Antigen Cd142 |
Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
Alternative ELISA Names | Tissue Factor ELISA kitTf ELISA kitCoagulation Factor Iii ELISA kitCd Antigen Cd142 ELISA kitF3 ELISA kitCf3 ELISA kit |
Specificity | This kit recognizes Rat TF in samples. No significant cross-reactivity or interference between Rat TF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 84-97 | 90 |
EDTA plasma (n=8) | 88-100 | 95 |
Cell culture media (n=8) | 87-101 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 92.92 | 228.98 | 963.73 | 99.52 | 234.39 | 1029.26 |
Standard deviation | 5.02 | 11.93 | 29.20 | 5.37 | 13.31 | 30.88 |
CV (%) | 5.40 | 5.21 | 3.03 | 5.40 | 5.68 | 3.00 |