| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.055ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This SLC16A1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | Slc16a1 |
| Gene ID: | 25027 |
| Uniprot ID: | MOT1_RAT |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Detected in erythrocytes (at protein level). Detected in brain, heart, kidney, lung, muscle, jejunum enterocytes and brain capillaries. |
| Post Translational Modifications | |
| Function | Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH. The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis. Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 302-Asp is essential for the conformational transition. |
| Protein Name | Monocarboxylate Transporter 1 Mct 1 Solute Carrier Family 16 Member 1 |
| Database Links | Reactome: R-RNO-210991 Reactome: -RNO-433692 Reactome: -RNO-9749641 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Apical Cell Membrane Expression At The Cell Surface Requires The Ancillary Proteins Bsg And Emb |
| Alternative ELISA Names | Monocarboxylate Transporter 1 ELISA kit Mct 1 ELISA kit Solute Carrier Family 16 Member 1 ELISA kit Slc16a1 ELISA kit Mct1 ELISA kit |
| output |
Information sourced from Uniprot.org
