| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.108ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description : | This Rock2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Rock2 |
| Gene ID: | 25537 |
| Uniprot ID: | ROCK2_RAT |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Highly expressed in brain, lung, liver, skeletal muscle, kidney and testis. |
| Post Translational Modifications | Autophosphorylated. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity. Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
| Function | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. |
| Protein Name | Rho-Associated Protein Kinase 2 Rho-Associated - Coiled-Coil-Containing Protein Kinase 2 Rho-Associated - Coiled-Coil-Containing Protein Kinase Ii Rock-Ii Rhoa-Binding Kinase 2 P150 Rok-Alpha Rokalpha P164 Rock-2 |
| Database Links | Reactome: R-RNO-3928662 Reactome: -RNO-416482 Reactome: -RNO-416572 Reactome: -RNO-4420097 Reactome: -RNO-5627117 Reactome: -RNO-8980692 Reactome: -RNO-9013026 Reactome: -RNO-9013407 Reactome: -RNO-9013422 |
| Cellular Localisation | Cytoplasm Cell Membrane Peripheral Membrane Protein Nucleus Cytoskeleton Microtubule Organizing Center Centrosome Cytoplasmic And Associated With Actin Microfilaments And The Plasma Membrane |
| Alternative ELISA Names | Rho-Associated Protein Kinase 2 ELISA kit Rho-Associated - Coiled-Coil-Containing Protein Kinase 2 ELISA kit Rho-Associated - Coiled-Coil-Containing Protein Kinase Ii ELISA kit Rock-Ii ELISA kit Rhoa-Binding Kinase 2 ELISA kit P150 Rok-Alpha ELISA kit Rokalpha ELISA kit P164 Rock-2 ELISA kit Rock2 ELISA kit |
| output |
Information sourced from Uniprot.org