| Host: |
Mouse/Mouse |
| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This OC/BGP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Purification: |
Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Determination Method: |
Colormetric |
| Gene Symbol: |
Bglap |
| Gene ID: |
25295 |
| Uniprot ID: |
OSTCN_RAT |
| Immunogen: |
Capture immunogen: Recombinant Rat OC/BGP protien.Detection Immunogen: Recombinant Rat OC/BGP protien. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein: it acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization. The carboxylated form binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. |
| Protein Name | OsteocalcinBone Gla ProteinBgpGamma-Carboxyglutamic Acid-Containing Protein |
| Database Links | Reactome: R-RNO-159740Reactome: -RNO-159763Reactome: -RNO-159782 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteocalcin antibody pairBone Gla Protein antibody pairBgp antibody pairGamma-Carboxyglutamic Acid-Containing Protein antibody pairBglap antibody pairBglap2 antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
