| Host: | Insect cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Rat Interleukin-12 p40-protein was developed from insect cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED (50) was determined by secretion of IFN-gamma fromPHA and IL-2 activated mouse splenocytes that have been costimulatedwith PMA, and wad found to be less than 0.05 ng/ml. NA |
| Immunogen Region: | Mature chain |
| Immunogen: | Optimized DNA sequence encoding rat Interleukin-12 mature chain was expressed in SF9 insect cells. NA |
| Immunogen Sequence: | NA |
| Background | Native rat Interleukin-12 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 70 kDa. Recombinant rat Interleukin-12 is a disulfide-linked heterodimeric protein, and migrates as an approximately 70 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Information sourced from Uniprot.org