| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This Il1r1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Il1r1 |
| Uniprot ID: |
IL1R1_RAT |
| Specificity: |
This kit recognizes Rat IL-1R1 in samples. No significant cross-reactivity or interference between Rat IL-1R1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells. |
| Protein Name | Interleukin-1 Receptor Type 1Il-1r-1Il-1rt-1Il-1rt1Cd121 Antigen-Like Family Member AInterleukin-1 Receptor AlphaIl-1r-AlphaInterleukin-1 Receptor Type IP80Cd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane FormMil-1r1Mil-1ri - Interleukin-1 Receptor Type 1 - Soluble FormSil-1r1Sil-1ri |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell MembraneSecreted |
| Alternative ELISA Names | Interleukin-1 Receptor Type 1 ELISA kitIl-1r-1 ELISA kitIl-1rt-1 ELISA kitIl-1rt1 ELISA kitCd121 Antigen-Like Family Member A ELISA kitInterleukin-1 Receptor Alpha ELISA kitIl-1r-Alpha ELISA kitInterleukin-1 Receptor Type I ELISA kitP80 ELISA kitCd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane Form ELISA kitMil-1r1 ELISA kitMil-1ri - Interleukin-1 Receptor Type 1 - Soluble Form ELISA kitSil-1r1 ELISA kitSil-1ri ELISA kitIl1r1 ELISA kitIl1ra ELISA kit |
| Specificity | This kit recognizes Rat IL-1R1 in samples. No significant cross-reactivity or interference between Rat IL-1R1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 102 |
| EDTA plasma (n=8) | 91-106 | 98 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 243.40 | 691.14 | 2333.41 | 234.33 | 715.02 | 2327.87 |
| Standard deviation | 13.09 | 39.26 | 101.50 | 16.00 | 34.61 | 111.74 |
| CV (%) | 5.38 | 5.68 | 4.35 | 6.83 | 4.84 | 4.80 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
