| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IFN-gamma R1 at 3 Mu g/mL (100 Mu L/well) can bind recombinant Rat IFNG, the EC50 of Rat IFNG is 1 Mu g/mL.|2.Measured by its binding ability in a funct |
| Gene Symbol: |
Ifng |
| Gene ID: |
25712 |
| Uniprot ID: |
IFNG_RAT |
| Immunogen Region: |
Gln23-Cys156 |
| Immunogen: |
Recombinant Rat IFN-gamma Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln23-Cys156) of rat Interferon Gamma (Accession #NP_620235.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: |
QGTLIESLESLKNYFNSSSM DAMEGKSLLLDIWRNWQKDG NTKILESQIISFYLRLFEVL KDNQAISNNISVIESHLITN FFSNSKAKKDAFMSIAKFEV NNPQIQHKAVNELIRVIHQL SPESSLRKRKRSRC |
| Tissue Specificity | Released primarily from activated T lymphocytes. |
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. |
| Protein Name | Interferon GammaIfn-Gamma |
| Database Links | Reactome: R-RNO-877300Reactome: -RNO-877312Reactome: -RNO-9732724 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interferon Gamma proteinIfn-Gamma proteinIfng protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
