| Function | Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. |
| Protein Name | Heme Oxygenase 1Ho-1Hsp32 Cleaved Into - Heme Oxygenase 1 Soluble Form |
| Database Links | Reactome: R-RNO-189483Reactome: -RNO-917937Reactome: -RNO-9609523Reactome: -RNO-9707564Reactome: -RNO-9707587 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinCytoplasmic Side |
| Alternative ELISA Names | Heme Oxygenase 1 ELISA kitHo-1 ELISA kitHsp32 Cleaved Into - Heme Oxygenase 1 Soluble Form ELISA kitHmox1 ELISA kit |
| Specificity | This kit recognizes Rat HO1 in samples. No significant cross-reactivity or interference between Rat HO1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-96 | 90 |
| EDTA plasma (n=8) | 90-103 | 98 |
| Cell culture media (n=8) | 88-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.51 | 1.37 | 4.10 | 0.46 | 1.37 | 4.04 |
| Standard deviation | 0.03 | 0.06 | 0.16 | 0.03 | 0.06 | 0.20 |
| CV (%) | 5.88 | 4.38 | 3.90 | 6.52 | 4.38 | 4.95 |
