| Short Description : | Recombinant-Rat Histone H3.3-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications: | ELISA/WB |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% SKL, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | Please contact us for this information. |
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H3.3 |
| Database Links | Reactome: R-RNO-212300Reactome: -RNO-2559580Reactome: -RNO-2559582Reactome: -RNO-427359Reactome: -RNO-427413Reactome: -RNO-5250924Reactome: -RNO-5578749Reactome: -RNO-5625886Reactome: -RNO-68616Reactome: -RNO-73728Reactome: -RNO-73772Reactome: -RNO-8936459Reactome: -RNO-9018519Reactome: -RNO-983231Reactome: -RNO-9841922Reactome: -RNO-9843940Reactome: -RNO-9843970 |
| Cellular Localisation | NucleusChromosome |
| Alternative Protein Names | Histone H3.3 proteinH3-3b proteinH3.3b proteinH3f3b protein |
Information sourced from Uniprot.org