| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat GFAP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Gfap |
| Gene ID: |
24387 |
| Uniprot ID: |
GFAP_RAT |
| Specificity: |
This kit recognizes Rat GFAP in samples. No significant cross-reactivity or interference between Rat GFAP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. |
| Post Translational Modifications | Phosphorylated by PKN1. |
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Protein Name | Glial Fibrillary Acidic ProteinGfap |
| Cellular Localisation | CytoplasmAssociated With Intermediate Filaments |
| Alternative CLIA Names | Glial Fibrillary Acidic Protein CLIA kitGfap CLIA kitGfap CLIA kit |
| Specificity | This kit recognizes Rat GFAP in samples. No significant cross-reactivity or interference between Rat GFAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-107 | 97 |
| EDTA plasma(n=8) | 84-100 | 91 |
| Cell culture media(n=8) | 101-117 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 198.82 | 517.36 | 1456.27 | 214.62 | 541.21 | 1491.35 |
| Standard deviation | 16.22 | 43.46 | 156.26 | 26.27 | 59.70 | 141.68 |
| CV (%) | 8.16 | 8.40 | 10.73 | 12.24 | 11.03 | 9.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
