This FASL Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma, tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Rat
Sensitivity
0.076ng/mL
Detection Limit
0.312-20ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates or other biological fluids.
Additional Info
Tissue Specificity
Expressed in activated splenocytes and thymocytes. Moderate or weak expression found in small intestines, kidney and lung.
Post Translational Modifications
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. Phosphorylated by FGR on tyrosine residues.this is required for ubiquitination and subsequent internalization. N-glycosylated. Monoubiquitinated.
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis. Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways. Can induce apoptosis but does not appear to be essential for this process. FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition.
Cell MembraneSingle-Pass Type Ii Membrane ProteinCytoplasmic Vesicle LumenLysosome LumenColocalizes With The Sppl2a Protease At The Cell MembraneIs Internalized Into Multivesicular Bodies Of Secretory Lysosomes After Phosphorylation By Fgr And MonoubiquitinationTumor Necrosis Factor Ligand Superfamily Member 6Soluble Form: SecretedMay Be Released Into The Extracellular Fluid By Cleavage From The Cell SurfaceFasl Intracellular Domain: NucleusThe Fasl Icd Cytoplasmic Form Is Translocated Into The Nucleus
