| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.117ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description : | This DIO3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | Dio3 |
| Gene ID: | 29475 |
| Uniprot ID: | IOD3_RAT |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | Neonatal skin, placenta, skeletal muscle and cerebral cortex. |
| Post Translational Modifications | |
| Function | Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,3',5'-triiodothyronine (rT3), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine (3-T1), rT3 to 3',5'-diiodothyronine (3',5'-T2) and 3,3'-diiodothyronine (3,3'-T2) to 3'-monoiodothyronine (3'-T1) via inner-ring deiodination (IRD). Catalyzes the deiodination of 3,5,3'-triiodothyronine (T3) to 3,3'-diiodothyronine (3,3'-T2) via IRD. Catalyzes the deiodination of 3-T1 to L-thyronine (T0) via outer-ring deiodination (ORD). Catalyzes the tyrosyl ring deiodinations of T4AM (3,3',5,5'-tetraiodothyronamine), rT3AM (3,3',5'-triiodothyronamine), T3AM (3,5,3'-triiodothyronamine), 3,5-T2AM (3,5-diiodothyronamine), 3,3'-T2AM (3,3'-diiodothyronamine) and 3-T1AM (3-iodothyronamine). |
| Protein Name | Thyroxine 5-Deiodinase5diiiDioiiiType 3 DiType Iii Iodothyronine Deiodinase |
| Database Links | Reactome: R-RNO-350864 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinEndosome Membrane |
| Alternative ELISA Names | Thyroxine 5-Deiodinase ELISA kit5diii ELISA kitDioiii ELISA kitType 3 Di ELISA kitType Iii Iodothyronine Deiodinase ELISA kitDio3 ELISA kitItdi3 ELISA kitTxdi3 ELISA kit |
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Information sourced from Uniprot.org
