Rat CD47 protein (Recombinant) (C-hFc) (STJP002873)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Rat CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 19-Lys 140) of rat CD47 (Accession #NP_062068.1) fused with and a hFc tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat CD47-C-hFc protein was developed in hek293 cells using the region Gln 19-Lys 140. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA.Immobilized Human CD172a at 2 Mu g/mL (100 Mu L/well) can bind Human CD47 with a linear range of 19.5-3491.5ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Cd47 |
| Gene ID | 29364 |
| Uniprot ID | CD47_RAT |
| Immunogen | Recombinant Rat CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 19-Lys 140) of rat CD47 (Accession #NP_062068.1) fused with and a hFc tag at the C-terminus. |
| Immunogen Region | Gln 19-Lys 140 |
| Immunogen Sequence | QLLLSKVKSVEFTSCNDTVV IPCKVLNVEAQSTDEMFVKW KLNKSYIFIYDGNKNSTTRE QNFTSAKISVSDLLKGIASL TMDTHEAVVGNYTCEVTELS REGKTVIELKNRPVSWFSTN EK |
Additional Info
| Function | Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering. Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging. In response to THBS1, negatively modulates wound healing. Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. |
| Protein Name | Leukocyte Surface Antigen Cd47Integrin-Associated ProteinIapCd Antigen Cd47 |
| Database Links | Reactome: R-RNO-202733Reactome: -RNO-216083Reactome: -RNO-391160Reactome: -RNO-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Protein Names | Leukocyte Surface Antigen Cd47 proteinIntegrin-Associated Protein proteinIap proteinCd Antigen Cd47 proteinCd47 protein |
Information sourced from Uniprot.org